Journal: Cell Death Discovery

Article Title: Targeting GPX2 to disrupt lipid homeostasis and enhance cisplatin sensitivity in diffuse gastric cancer

doi: 10.1038/s41420-025-02771-8

Figure Lengend Snippet: A UMAP plot of GPX2-positive and GPX2-negative subsets of malignant gastric epithelial cells. B Results of gene set variation analysis (GSVA) for GPX2-positive and GPX2-negative cells. C The GSEA plot of differentially expressed genes between GPX2-positive and GPX2-negative cells. D Violin plot illustrating the expression of lipid metabolism markers in GPX2-positive and GPX2-negative subsets. E Time-course analysis of GPX2 protein levels in AGS and BGC823 cells stimulated with 200 μM oleic acid for 0, 12, 24, and 48 h. F Western blot analysis of lipid metabolism-related gene expression in GPX2-knockdown AGS and GPX2-overexpressed BGC823 cells. G BODIPY 493/503 fluorescence staining of GPX2-knockdown AGS and SNU601 cells, stimulated by 200 μM oleic acid for 24 h, illustrating the impact on lipid droplet production. Scale bars = 50 μm. H BODIPY 493/503 fluorescent dye was used to visualize lipid droplets in the GPX2-overexpressed BGC823 and HGC27 cells. Scale: 50 μm.The data are represented as the mean ± SD of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001, ns no significance.

Article Snippet: For BODIPY493/503 staining: 100 μL of a 10 μM BODIPY 493/503 working solution (MCE, NJ, USA) was added to each well for a staining duration of 30 min.

Techniques: Expressing, Western Blot, Gene Expression, Knockdown, Fluorescence, Staining